ACTIVITIES

• General objects:
  
  • Bioactive peptides
    
  • Synthesis
    
  • Isolation
    
  • Labelling
    
  • Physicochemical characterization
    
  • Βiological Evaluation
    
  • Αntibodies
    
  • Development in mammalian and avian species
    
  • Isolation from blood serum or egg yolk
    
  • Labelling
    
  • Immunochemical characterization
    
  • Immunodiagnosis / Immunotherapy
    
  • Immunochemical diagnostic techniques (e.g. RIA, IRMA, ELISA, immunohistochemistry)
    
  • Development
    
  • Standarization
    
  • Clinical Evaluation
• Main current activities
  
  • Study of thymosin bioactive peptides as means of disease diagnosis / therapy.
    
  • Development of immunohistochemical techniques for diagnosis / prognosis of breast cancer.
    
  • Development and clinical evaluation of an immunoenzymometric assay for determining Thyroid Stimulating Hormone (TSH) in dried blood spots for the Screening Programme for Congenital Hypothyroidism among Greek neonates.
    
  • Development of immunochemical analytical techniques for the early detection of diseases due to working conditions.
  • Development of vaccines based on copolymers of poly (lactic-co-glycolic)acid for the regulation of Th1/Th2 immunoresponse.
    
  • Design, synthesis and immunochemical evaluation of dendrimers with antigenic and immunogenic properties.
    

ACCESSIBLE INFRASTRUCTURE

• ESI- mass spectrometer connected with HPLC (Finnigan, AQA Navigator; Waters, Pump Alliance 2690 UV 2487).
  
• Αmino acid analyzer - analytical HPLC (Waters, Pico Tag, UV Detector: 996, Pump: 616)
  
• Semi-preparative HPLC system (Waters, UV Detector: 996, Pump: 600S; Waters, UV Detector: 484, Pump: 600E).
  
• Cooling Centrifuge (Sorvall, RC5C)
  
• CO2 incubator (Forma scientific, 3546/3548).
  
• Laminar flow chamber, class 10 and 100 (ELMED).
  
• Shaker incubator for cell cultures.
  
• γ-Counter (Oakfield, Sourcerer).
  
• ELISA reader (Dynatech, MR 5000).
  
• System for the shaking, washing and dispensing of liquids in ELISA microplates (STL, EAS 2/4; Denley, Wellwash 4; Denley, Wellfill 5).
  
• Optical Microscope (Nikon, E400) with digital depiction of images (JVC, TK-C1381) on a PC.
  
• Μicrotome (Leica, RM2135).

SERVICES/PRODUCTS PROVIDED
A novel, non-radioisotopic method for the detection of TSH levels in dry blood spots is under clinical evaluation, as part of a program financed by the GSRT and the National Institute of Children's Health ("end user" partner). The new method is expected to replace the existing radioisotopic methodology that is used up to now in the Screening Programme for the Detection of Congenital Hypothyroidism in Greek neonates.

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ACTIVITIES
DNA sequencing
protein expression in vitro

ACCESSIBLE INFRASTRUCTURE
GENETIC ANALYSER PE-ABI310 (DNA SEQUENCER)

SERVICE PROVIDED/PRODUCTS AVAILABLE
DNA sequencing
protein expression

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ACTIVITIES

• Biocalorimetry
  
• Isothermal titration/Microcalorimetry
  
• Differential Scanning Microcalorimetry
  
• High-resolution x-ray scattering
  
• Molecular self-assembly
  
• Lipid-based supermolecular systems
  
• Biomolecular interactions
• Structure-based thermodynamics of macromolecules in solutions
• Critical phenomena

ACCESSIBLE INFRASTRUCTURE

• VP-DSC
  
• MCS-ITC
  
• Cecullar Dichroism
  
• X-ray Crystallography

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ACTIVITIES

• Research, Development and Preparation of certified reference materials for immunoassay.
  
• Research and Development of immunoassay of tumor markers e.g. PSA.
  
• Research and Development of Immobilisation of antibodies-antigens in solid phase.
  
• Research and Development of immunoassay of free hormones, e.g. FT4
  
• Research: ligands-free molecules in human serum

ACCESSIBLE INFRASTRUCTURE

• ELISA READER
  
• Fluorescence Plate Reader
  
• HPLC amino acid analyser
  
• Electrophoresis systems
  
• γ. counter

SERVICE PROVIDED/PRODUCTS AVAILABLE

• Production and Quality Control of immunodiagnostic products.
  
• External Quality Assessment of Immunoassays.
  
• Quality Assessment of Imported Immunoassay Reagents

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ACTIVITIES

• In-vivo investigation of the biodistribution of new radiolabelled compounds and radiopharmaceuticals. Structure-activity relationship (SAR)
  
• Animal models for the evaluation of new radiopharmaceuticals
  
• Tumor transplantation in animals for the evaluation of new radiopharmaceuticals to be tested in oncology
  
• Trials of radiopharmaceuticals for clinical use
  
• Labeling and in-vivo evaluation of biomolecules (monoclonal antibodies and peptides) to be used in nuclear medicine

ACCESSIBLE INFRASTRUCTURE

• Shielded hot-cell
  
• Laminar flow unit
  
• Biodistribution studies laboratory

SERVICE PROVIDED/PRODUCTS AVAILABLE
Biological control of radiopharmaceuticals for clinical use
Radiopharmaceuticals

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ACTIVITIES

• Development of optical immunosensors using conventional solid materials.
  
• Development of optical immunosensors using Si-based material. (chips sensors).
  
• R&D on new activated material for use in immunosensors and conventional immunoassay.
  
• Multianalyte immunosensors.

ACCESSIBLE INFRASTRUCTURE

• Optoelectronic set up for capillary sensors
  
• ELISA reader.
  
• Fluorescence Plate Reader.
  
• HPLC amino acid analyser.
  
• Electrophoresis systems.
  
• γ. counter

SERVICE PROVIDED/PRODUCTS AVAILABLE

• Immunodiagnostic products
  
• Customer oriented development of specific methodologies
  

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ACTIVITIES
Radiochemistry, Radioisotopes, Radiopharmaceuticals, Diagnosis and Therapy, Radioscintigraphy, Radioimmunodiagnosis and Therapy.
Labeled Biomolecules (peptides, antibodies)
Quality Control of Radiopharmaceuticals.

ACCESSIBLE INFRASTRUCTURE

• Nuclear measurements (γ-spectroscopy)
  
• Total radioactivity measurement.
  
• Electronic autoradiography.
  
• Radiochromatographic scanners.
  
• HPLC
  
• Conventional analytical chromatographic technologies.
  
• Auroradiography.

SERVICE PROVIDED/PRODUCTS AVAILABLE
Quality Control of Radiopharmaceuticals (imported or produced in Greece)
Pre-marketing and Post-marketing Quality Control.

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ACTIVITIES

• Synthesis of organic compounds.
  
• Coordination compounds.
  
• Characterization of synthesised compounds.
  
• Synthesis of labeling compounds and their characterisation.
  
• Pharmacological evaluation in-vivo & in-vitro.

ACCESSIBLE INFRASTRUCTURE

• Lyophilisator
  
• Hot cell β-γ
  
• Clan Laboratory calls 100
  
• Elemental analyser IR, UV
  
• HPLC coupled to UV and rad. Detector.

SERVICE PROVIDED/PRODUCTS AVAILABLE
PRODUCTS

• Four (4) iodine [131I] pharmaceutical preparations - two (2) in the form of oral solutions and two (2) in the form of oral capsules - for diagnosis and therapy of thyroid diseases
• Technetium-99m in the form of sodium pertechnetate-[99mTc] injection solution
• Nine (9) technetium-99m cold kits for the preparation of radiopharmaceutical complexes for the scintigraphy of various tumors and diseases (bone scanning, static and dynamic renal imaging, thyroid medullary carcinoma imaging, hepatobiliary system imaging, liver and kidneys scintigraphy, myocardium imaging, red cell labelling, thyroid gland tumors imaging, e.t.c.)
  

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